Re-bound at full text: ASH2L still has no haematopoietic cell-state result - the loose intersection is 429 records and the tight one 17, and every title read is a review, a complex-membership mention or a congress abstract book
also called Q115 ASH2L cell-state re-bound
Nobody has shown what cell condition ASH2L holds a blood cell in, and reading the whole text of papers rather than only their summaries does not turn anyone up. What the search returns is review articles that list ASH2L as part of a protein complex.
The same thing, for a scientist
`absence-no-cell-state-for-ash2l` bounds its claim with a `[tiab]` query against an 18-term cell-state clause, which rule 80 says is not a bound. Re-run at full text with aliases `ASH2L OR ASH2L1`: the alias group alone returns **1437**, crossed with the full state clause and a leukaemia clause **429**, and with a narrow state clause **356** - all too large to read [E1]. **The tractable form is the tight phrase intersection: `ASH2L x "leukemia stem cell"` returns **17**, and every one of those titles was read** [E1]. The composition is uniform and is the result: family and epigenetics reviews, papers in which ASH2L is named as a member of a complex rather than studied, and congress abstract books. **No primary study places ASH2L in a haematopoietic cell state. The absence survives.** In DepMap the KMT2A-rearranged arm is +0.157 relative to all lines - the only positive non-floor-compressed contrast in that beat's worklist - so the dependency data point away from a fusion-specific requirement.
The records that came closest, and why none of them is the answer
- PMID 20093773, c-Myb binds MLL through menin in human leukemia cells and is an important driver of MLL-associated leukemogenesis., J Clin Invest 2010 - a menin-MLL-cMyb driver paper; ASH2L appears as a complex member, no state measurement
- PMID 35338774, Functional mapping of PHF6 complexes in chromatin remodeling, replication dynamics, and DNA repair., Blood 2022 - PHF6 complex mapping; ASH2L as an interactor
- PMID 39342993, Unraveling MLL1-fusion leukemia: Epigenetic revelations from an iPS cell point mutation., J Biol Chem 2024 - an iPS point-mutation study of MLL1 fusion, not an ASH2L state result
The pattern across all four genes re-bounded this way is the same: a full-text search for a complex subunit returns the complex's literature, not the subunit's. That is a structural property of how these proteins are written about, and it means the loose intersection can never bound a subunit-level claim however large the corpus is.
| script | none - Europe PMC BODY: queries, 2026-09-22 |
|---|---|
| status | run |
| verdict | SURVIVES at full text on the tight query; the loose intersections are unscreened upper bounds |
| entry re bounded | absence-no-cell-state-for-ash2l |
| instrument before | abstract-level query against an 18-term cell-state clause |
| instrument after | full-text tight phrase intersection, all titles read, over 12,362,145 full texts |
| dependency context | In DepMap the KMT2A-rearranged arm is +0.157 relative to all lines - the only positive non-floor-compressed contrast in that beat's worklist - so the dependency data point away from a fusion-specific requirement. |
| structural finding | A full-text search for a complex SUBUNIT returns the COMPLEX's literature. The loose intersection cannot bound a subunit-level claim at any corpus size, which is why only the tight phrase form is reported as a screened zero. |
| absence class | measured-not-analysed |
| absence class basis | UNCHANGED from the 2026-09-21 assignment: GEO holds 760 human bone-marrow single-cell RNA-seq DataSets and GSE120221 was verified individually, which closes the DESCRIPTIVE half (which populations express the gene and whether that tracks a state) and leaves the FUNCTIONAL half never-measured. This re-bound changes the literature instrument only. |
queries — the numbers behind the chart
[
{
"q": "ASH2L alias group",
"hits": 1437,
"role": "operand control"
},
{
"q": "alias group AND 11-term state clause AND leukaemia clause",
"hits": 429,
"screened": false
},
{
"q": "alias group AND narrow state clause AND leukaemia clause",
"hits": 356,
"screened": false
},
{
"q": "ASH2L x \"leukemia stem cell\"",
"hits": 17,
"read": 17,
"on_topic": 0
}
]aliases searched
[ "ASH2L", "ASH2L1" ]
nearest records — the numbers behind the chart
[
{
"pmid": "20093773",
"title": "c-Myb binds MLL through menin in human leukemia cells and is an important driver of MLL-associated leukemogenesis.",
"venue": "J Clin Invest",
"year": 2010,
"why_not": "a menin-MLL-cMyb driver paper; ASH2L appears as a complex member, no state measurement"
},
{
"pmid": "35338774",
"title": "Functional mapping of PHF6 complexes in chromatin remodeling, replication dynamics, and DNA repair.",
"venue": "Blood",
"year": 2022,
"why_not": "PHF6 complex mapping; ASH2L as an interactor"
},
{
"pmid": "39342993",
"title": "Unraveling MLL1-fusion leukemia: Epigenetic revelations from an iPS cell point mutation.",
"venue": "J Biol Chem",
"year": 2024,
"why_not": "an iPS point-mutation study of MLL1 fusion, not an ASH2L state result"
}
]- The 429-record loose intersection was never screened. Reading it is the only way to bound this claim over the full 18-term state vocabulary the entry uses.
- The descriptive half is a desk job on GSE120221 and 759 other bone-marrow single-cell datasets, and it has been a desk job since 2026-09-21. Which haematopoietic populations express ASH2L, and does expression track quiescence versus cycling?
- Does any of the three nearest records contain a ASH2L-specific perturbation in a defined state, in a supporting figure? None was opened.
- E1strong confidence 4 of 5: Replicated in peer-reviewed work by more than one group.database
At full text the ASH2L alias group returns 1437 records alone, 429 crossed with a cell-state clause and a leukaemia clause, 356 with a narrow state clause, and 17 in the tight phrase intersection - all of which were read and none of which is a haematopoietic cell-state result for ASH2L.
What this evidence cannot show
Europe PMC BODY: reaches only the 12,362,145 full texts the index holds. A congress abstract book is one document holding thousands of unrelated abstracts and matches almost any conjunction; across this beat's 18 fully-read intersections 83 of 321 records (25.9%, range 0-50%) are proceedings. Screening was at TITLE level throughout this entry. Only the tight intersection was read in full; the 429-record and 356-record intersections were counted and NOT screened, so this re-bound rests on the tight query alone. The tight query uses one or two state phrases, so a paper reporting a different state - dormancy, a drug-tolerant persister state, a differentiation block - without those phrases would not be reached.
Evidence that argues against this (1)
- A tight phrase query trades recall for readability and this one keeps only 'leukemia stem cell' and, where run, 'quiescence'. The entry under test used an 18-term state clause for exactly that reason. So the honest statement is that the tight intersection is a SCREENED zero over a narrow state vocabulary, sitting inside an unscreened loose intersection of 429 records - which is better than an abstract-level zero and is not a bound over the whole state vocabulary. Europe PMC 2026
SURVIVES at full text: the tight phrase intersection (17 records) was read in full and contains no haematopoietic cell-state result
- related to →ASH2L — the WRAD subunit that has DPY30's essentiality profile and none of its co-dependency signal
no full-text record places this protein in a haematopoietic cell state; the recall is its complex's literature